Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor
Identifieur interne : 000009 ( OpenAccess/Analysis ); précédent : 000008; suivant : 000010Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor
Auteurs : Nora Freyer ; Fanny Knöspel ; Nadja Strahl ; Leila Amini ; Petra Schrade ; Sebastian Bachmann ; Georg Damm ; Daniel Seehofer ; Frank Jacobs ; Mario Monshouwer ; Katrin ZeilingerSource :
- BioResearch Open Access [ 2164-7844 ] ; 2016.
Abstract
The hepatic differentiation of human induced pluripotent stem cells (hiPSC) holds great potential for application in regenerative medicine, pharmacological drug screening, and toxicity testing. However, full maturation of hiPSC into functional hepatocytes has not yet been achieved. In this study, we investigated the potential of a dynamic three-dimensional (3D) hollow fiber membrane bioreactor technology to improve the hepatic differentiation of hiPSC in comparison to static two-dimensional (2D) cultures. A total of 100 × 106 hiPSC were seeded into each 3D bioreactor (
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DOI: 10.1089/biores.2016.0027
PubMed: 27610270
PubMed Central: 5003005
Affiliations:
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sort="Schrade, Petra" uniqKey="Schrade P" first="Petra" last="Schrade">Petra Schrade</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Bachmann, Sebastian" sort="Bachmann, Sebastian" uniqKey="Bachmann S" first="Sebastian" last="Bachmann">Sebastian Bachmann</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Damm, Georg" sort="Damm, Georg" uniqKey="Damm G" first="Georg" last="Damm">Georg Damm</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Seehofer, Daniel" sort="Seehofer, Daniel" uniqKey="Seehofer D" first="Daniel" last="Seehofer">Daniel Seehofer</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Jacobs, Frank" sort="Jacobs, Frank" uniqKey="Jacobs F" first="Frank" last="Jacobs">Frank Jacobs</name><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Monshouwer, Mario" sort="Monshouwer, Mario" uniqKey="Monshouwer M" first="Mario" last="Monshouwer">Mario Monshouwer</name><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Zeilinger, Katrin" sort="Zeilinger, Katrin" uniqKey="Zeilinger K" first="Katrin" last="Zeilinger">Katrin Zeilinger</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27610270</idno><idno type="pmc">5003005</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003005</idno><idno type="RBID">PMC:5003005</idno><idno type="doi">10.1089/biores.2016.0027</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000339</idno><idno type="wicri:Area/Pmc/Curation">000339</idno><idno type="wicri:Area/Pmc/Checkpoint">000043</idno><idno 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Knöspel</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Strahl, Nadja" sort="Strahl, Nadja" uniqKey="Strahl N" first="Nadja" last="Strahl">Nadja Strahl</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Amini, Leila" sort="Amini, Leila" uniqKey="Amini L" first="Leila" last="Amini">Leila Amini</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Schrade, Petra" sort="Schrade, Petra" uniqKey="Schrade P" first="Petra" last="Schrade">Petra Schrade</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Bachmann, Sebastian" sort="Bachmann, Sebastian" uniqKey="Bachmann S" first="Sebastian" last="Bachmann">Sebastian Bachmann</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Damm, Georg" sort="Damm, Georg" uniqKey="Damm G" first="Georg" last="Damm">Georg Damm</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Seehofer, Daniel" sort="Seehofer, Daniel" uniqKey="Seehofer D" first="Daniel" last="Seehofer">Daniel Seehofer</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Jacobs, Frank" sort="Jacobs, Frank" uniqKey="Jacobs F" first="Frank" last="Jacobs">Frank Jacobs</name><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Monshouwer, Mario" sort="Monshouwer, Mario" uniqKey="Monshouwer M" first="Mario" last="Monshouwer">Mario Monshouwer</name><affiliation><nlm:aff id="aff5"></nlm:aff></affiliation></author><author><name sortKey="Zeilinger, Katrin" sort="Zeilinger, Katrin" uniqKey="Zeilinger K" first="Katrin" last="Zeilinger">Katrin Zeilinger</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BioResearch Open Access</title><idno type="ISSN">2164-7844</idno><idno type="eISSN">2164-7860</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>The hepatic differentiation of human induced pluripotent stem cells (hiPSC) holds great potential for application in regenerative medicine, pharmacological drug screening, and toxicity testing. However, full maturation of hiPSC into functional hepatocytes has not yet been achieved. In this study, we investigated the potential of a dynamic three-dimensional (3D) hollow fiber membrane bioreactor technology to improve the hepatic differentiation of hiPSC in comparison to static two-dimensional (2D) cultures. A total of 100 × 10<sup>6</sup> hiPSC were seeded into each 3D bioreactor (<italic>n</italic> = 3). Differentiation into definitive endoderm (DE) was induced by adding activin A, Wnt3a, and sodium butyrate to the culture medium. For further maturation, hepatocyte growth factor and oncostatin M were added. The same differentiation protocol was applied to hiPSC maintained in 2D cultures. Secretion of alpha-fetoprotein (AFP), a marker for DE, was significantly (<italic>p</italic> < 0.05) higher in 2D cultures, while secretion of albumin, a typical characteristic for mature hepatocytes, was higher after hepatic differentiation of hiPSC in 3D bioreactors. Functional analysis of multiple cytochrome P450 (CYP) isoenzymes showed activity of CYP1A2, CYP2B6, and CYP3A4 in both groups, although at a lower level compared to primary human hepatocytes (PHH). CYP2B6 activities were significantly (<italic>p</italic> < 0.05) higher in 3D bioreactors compared with 2D cultures, which is in line with results from gene expression. Immunofluorescence staining showed that the majority of cells was positive for albumin, cytokeratin 18 (CK18), and hepatocyte nuclear factor 4-alpha (HNF4A) at the end of the differentiation process. In addition, cytokeratin 19 (CK19) staining revealed the formation of bile duct-like structures in 3D bioreactors similar to native liver tissue. The results indicate a better maturation of hiPSC in the 3D bioreactor system compared to 2D cultures and emphasize the potential of dynamic 3D culture systems in stem cell differentiation approaches for improved formation of differentiated tissue structures.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kola, I" uniqKey="Kola I">I Kola</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lewis, Df" uniqKey="Lewis D">DF Lewis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giri, S" uniqKey="Giri S">S Giri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ingelman Sundberg, M" uniqKey="Ingelman Sundberg M">M Ingelman-Sundberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takayama, K" uniqKey="Takayama K">K Takayama</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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sortKey="Bachmann, Sebastian" sort="Bachmann, Sebastian" uniqKey="Bachmann S" first="Sebastian" last="Bachmann">Sebastian Bachmann</name><name sortKey="Damm, Georg" sort="Damm, Georg" uniqKey="Damm G" first="Georg" last="Damm">Georg Damm</name><name sortKey="Freyer, Nora" sort="Freyer, Nora" uniqKey="Freyer N" first="Nora" last="Freyer">Nora Freyer</name><name sortKey="Jacobs, Frank" sort="Jacobs, Frank" uniqKey="Jacobs F" first="Frank" last="Jacobs">Frank Jacobs</name><name sortKey="Knospel, Fanny" sort="Knospel, Fanny" uniqKey="Knospel F" first="Fanny" last="Knöspel">Fanny Knöspel</name><name sortKey="Monshouwer, Mario" sort="Monshouwer, Mario" uniqKey="Monshouwer M" first="Mario" last="Monshouwer">Mario Monshouwer</name><name sortKey="Schrade, Petra" sort="Schrade, Petra" uniqKey="Schrade P" first="Petra" last="Schrade">Petra Schrade</name><name sortKey="Seehofer, Daniel" sort="Seehofer, Daniel" uniqKey="Seehofer D" first="Daniel" last="Seehofer">Daniel Seehofer</name><name sortKey="Strahl, Nadja" sort="Strahl, Nadja" uniqKey="Strahl N" first="Nadja" last="Strahl">Nadja Strahl</name><name sortKey="Zeilinger, Katrin" sort="Zeilinger, Katrin" uniqKey="Zeilinger K" first="Katrin" last="Zeilinger">Katrin Zeilinger</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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